DNA

Part:BBa_K4115009:Design

Designed by: Renjie Yu   Group: iGEM22_ShanghaiTech_China   (2022-09-21)


NS3-2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 507
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NS3-2 and NSI-3-1 are a pair of commonly used homology arms in S.elongatus.


Source

The sequence of NS3-2 is gained from a NS3-targeting plasmid [1] and the fragment we use is artificially synthesized by the company.

References

[1] Lin PC, Zhang F, Pakrasi HB. Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973. Sci Rep. 2020 Jan 15;10(1):390. doi: 10.1038/s41598-019-57319-5. PMID: 31942010; PMCID: PMC6962321.